B.Sc. (Hons.) (Bio-Technology) 4th Semester
Plant Biotechnology
Paper : BIOT-Sem-IV-III-T
Time Allowed: Three Hours] [Maximum Marks: 67
Note: Attempt five questions in all by selecting one question from each Unit. Section A is compulsory. All questions carry equal marks except Question No.1.
1. Compulsory Question
(a) What are reporter genes?
(b) What are opines?
(c) What are virulence genes?
(d) What is Golden rice?
(e) What is T-DNA?
(f) Glyphosate resistant plants.
(g) Differentiate between stable and transient expression.
UNIT-I
2. (a) Give the differences between chloroplast and mitochondrial DNA.
(b) Describe in detail the various types of promoters used in plant gene expression for both dicots and monocots.
3. (a) Differentiate between :
(i) Selectable and Scorable marker
(ii) Binary and cointegrate vectors.
(b) What are repetitive sequences, exons, introns, and promoters?
UNIT-II
4. (a) Give the advantages and disadvantages of particle bombardment gene delivery method in plants.
(b) How are transgenic plants generated and maintained?
5. (a) Agrobacterium is a natural plant engineer. Justify the statement.
(b) Write a note on Antisense technology. SI5
UNIT-III
6. (a) What are the various genes which can be inserted to develop fungal and bacterial resistant plants?
(b) Name the organisms which are sources of transgene in golden rice and how was it developed?
7. (a) What is "Round up Maize"? How was it developed?
7. (b) Give the source of Bt genes and their mode of action to make plants resistant against insects.
UNIT-IV
8. (a) Define Biopharming. How are plants used as factories to develop antibodies?
(b) Write short notes on:
(i) Terpenoids
(ii) Flavanoids
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